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MICROSATELLITE AND ALLOZYME VARIATIONS IN STARLET STURGEON WILD BROODSTOCK AND HATCHERY-PRODUCED OFFSPRING, USED FOR RESTOCKING OF LOWER DANUBE RIVER

Abstract

Petya P. Ivanova, Nina S. Dzhembekova, Velichka Kardjeva, Angel G. Tsekov, Violin S. Raykov

The natural population of all sturgeon species has been seriously affected by overexploitation in combination with a substantial loss and degradation of habitats during the 20th century. Fish species undergoing to restocking require information about the genetic identity of the existing fish populations. The genetic pattern of sterlet (broodstock and offspring) were analyzed, using allozymes and seven microsatellite loci (LS19, LS34, LS39, LS54, LS68, Aox45 and Aox27) as markers for species identification. From the seven microsatellite loci three (LS57, Aox23 and Aox45) have shown a tetrasomic profile. The most polymorphic locus for the broodstock was LS-57 with 12 alleles in population, followed by Aox45, LS-68 and LS-54 with 10 alleles. For offspring, the most polymorphic locus was Aox 23 with 11 alleles. Other loci presented a lower level of polymorphism range between 2 to 8 alleles identified for the analyzed individuals. The average observed and expected heterozygosities were 0.429 and 0.413 in the broodstock and 0.500 and 0.423 in the offspring, respectively. The preliminary data showed that selected allozymes and microsatellite markers allow identification of the stellate sturgeon from Bulgarian farms and could be applied to test and control the broodstocks used for restocking programmes.

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